Note: items are listed in order of gameplay.
1st Location
Right after you examine the mummy statue in the Underground Temple, turn right then examine a small opened window on the wall. See: Part 1: Reunion and Separation
2nd Location
After killing the boss Osric, a hallway will open allowing you continue to the next area. You will notice a few jars in the corner while walking through the hallway. Destroy the jars and examine the ground. It's there, you just can't see it. See: Part 2: The Keep
3rd Location
Right after a cutscene with a boy and Kaede in front of a double doors sealed with weird green-looking plates, proceed to the next room with a staircase and a treasure box nearby. Above the treasure box is the Fluorite, you will have to stand close on the left of the treasure and press X to examine the wall. See: Part 3: Trap, Trap, Trap!
4th Location
This Fluorite is also hidden under a staircase. It is in a room on the second floor of the Keep's hall. See: Part 3: Trap, Trap, Trap!
5th Location
When you reach a storyline where you have to find 2 Crest Pieces to unlock a door. In the next room, you will trigger a cutscene with Hideyoshi Toyotomi. After that, continue to the screen, you will notice two tables on the sides, Fluorite is on the left screen (Samanosuke's right). See: Part 4: Rescuing the boy
6th Location
After you obtained Purifier Bell, go back to open the door that is blocked by spirits with the bell. Keep proceeding until you reach a corner of the last Samurai encased in a glass box. Press X to examine the corner. See: Part 5: Keep Underground
7th Location
The 7th Fluorite is in an iron maiden cage inside a room at the very end of the Underground Keep. See: Part 5: Keep Underground
8th Location
While playing as Kaede, you first objective is to obtain Wooden Gear to open a secret room that contain Silver Plate. Inside the same room, you will notice a statue in front of a red treasure box. Examine the statue to obtain the 8th Fluorite. See: Part 6: Found the Princess
9th Location
As Kaede, after you have collected both Silver and Gold Plates to open the gate, you will immediately notice the hallway is under fire so don't panic yet. To the left (Kaede's left) is another door, enter it. Fluorite is on a barrel next to the staircase. See: Part 6: Found the Princess
10th, 11th, 12th Locations
Three Fluorites are in the Dark Realm. The floors where they situated can be different depending on where you enter the Dark Realm (from the cocoon man in the sentry room or in the well). See: Part 7: Dark Realm
13th Location
The 10th location of Fluorite is on the ground very close to double doors sealed by level-3 blue crystal in the west area. You can access the east area after you found the Evil Plate and open the Evil door in the Keep. See: Part 8: From West to East
14th Location
It is under a catapult. To move the catapult, you have to cut the rope that tie it the fence. The catapult will roll downward and kill those zombies below and reveal the Fluorite underneath. See: Part 8: From West to East
15th Location
After you defeated the giant red ogre and picked up Decorated Sword, you next objective is to go back to the Keep's courtyard, east area. Along the way, you will see Fluorite on the ground flickering (assuming you have Vision Staff in your possession). See: Part 8: From West to East
17th Location
This one is very obvious since it is very noticeable if you have Vision Staff. It is located at a dock under a storage house near the waterfall. See: Part 8: From West to East
18th Location
While in control of Kaede for the second time, go straight and unlock the first door then turn right. Enter the room you see and pick up Fluorite on the ground. See: Part 9: A Helpful Partner
19th Location
Playing as Kaede, continue your way to the storage room near waterfall. Don't go down stairs to the dock yet. As Kaede, you can unlock the other door with Shinobi-Kit (where Samanosuke cannot previously). Continue your way until you are in room with 5 blue porcelain jars, turn right (Kaede's right) to enter the next area. There, you will see a Magic Mirror and a ladder. Claim up then destroy those crates to reveal the Fluorite on the floor. See: Part 9: A Helpful Partner
20th Location
Started from the 16th location, proceed to the area and prepare to block 2 arrows from Zombie Archers. Keep walking and turn right to the second door. Inside, you will find another ladder and some chests on the right side of the room. On top of them is the last Fluorite. See: Part 9: A Helpful Partner